Rhesus macaque genome study enhances our understanding of human genetic variation

Researchers at Baylor College of Medicine, the University of Missouri and the University of Washington have taken a major step toward improving our understanding of the genetic variation in the rhesus macaque, the most widely studied nonhuman primate in biomedical research.

Rhesus macaque is the most widely studied nonhuman primate in biomedical research. Image by Timothy Gonsalves.

“We have actually identified thousands of new mutations in the population of research animals. Now colleagues all over the country who are investigating various aspects of health and disease using rhesus macaques can begin to make use of that information,” said Dr. Jeffrey Rogers, associate professor at the Human Genome Sequencing Center and Department of Molecular and Human Genetics at Baylor and one of the corresponding authors of the study.

Dr. Jeffrey Rogers

Dr. Wesley C. Warren at the University of Missouri and Dr. Evan Eichler at the University of Washington, both corresponding authors of the study, used a combination of several advanced technologies to substantially improve the rhesus macaque reference genome assembly first created in 2007.

The new reference genome published in Science provides a more complete and accurate picture of the rhesus macaque DNA sequence by assembling and identifying more than 85 million genetic variants, the largest database of genetic variation for any one nonhuman primate species to date. This improvement can support more sophisticated and more detailed analyses of fundamental questions in molecular genetics, cell biology and physiology.

New exciting discoveries

“When we coupled this higher quality genome reference with a broader sequencing of expressed genes, our team discovered gene structures unique to macaque as well as specific repeat sequences undergoing deletions that suggest evolutionary competition to suppress these elements from expanding continues today,” said Warren, professor at the University of Missouri’s Bond Life Sciences Center.

Researchers at Baylor’s Human Genome Sequencing Center sequenced the genomes of 853 rhesus macaque from research institutions around the country and compared them to the new reference genome.

The genome analysis showed that rhesus macaques have more genetic variants per individual than humans. Among the millions of genetic variants identified, researchers found several damaging mutations in genes known to cause genetic disorders in humans, including autism, inherited blindness and several others.

“We can find naturally occurring models of genetic disorders by surveying the rhesus macaque population,” Rogers said. “We will find animals that naturally carry interesting and useful genetic mutations that can help us understand genetic variation and susceptibility to disease in humans. Rhesus macaques are also widely studied by primatologists and evolutionary biologists, so this new reference genome will also provide new insight into the evolution of the nonhuman primate and human genomes.”

For a full list of authors and funding, see the publication.

 

By Molly Chiu

 

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