Protein Beclin-1 is key for uterine remodeling and the establishment of pregnancy
Throughout a woman’s reproductive life, the endometrium, the mucous membrane lining the uterus, goes through cyclical remodeling. It thickens during the menstrual cycle in preparation for embryo implantation, and it is shed during menstruation when there is no fertilization.
Researchers at Baylor College of Medicine and collaborating institutions are investigating little-known factors directing uterine remodeling to advance the understanding of this process and provide new insights into fertility-associated gynecological conditions.
“One of our main goals is to understand biological processes that might be associated with pregnancy loss,” said corresponding author Dr. Rama Kommagani, associate professor in the Departments of Pathology and Immunology and Molecular Virology and Microbiology at Baylor. “In this study, we looked to understand the role Beclin-1 plays in endometrial reprogramming for the successful establishment of pregnancy.”
Beclin-1 and pregnancy
In the first part of this study published in the journal Developmental Cell, Kommagani and his colleagues found that Beclin-1 is essential for the establishment of pregnancy in mouse models. When they removed Beclin-1 specifically from uterine cells, the uteri did not develop properly, and this led to infertility caused by reduced uterine receptivity and failed embryo implantation.
In addition, molecular analysis of uteri revealed that Beclin-1 is necessary for endometrial programming of stem cells that leads to the development of uterine glands and other structures that are essential for uterine maturation.
“We also studied the mechanism by which Beclin-1 regulates uterine development,” Kommagani said.
Beclin-1 is known to regulate two distinct natural processes called autophagy and apoptosis that help maintain many normal functions in organisms. We investigated whether one of these two processes or both were involved.”
The team found that disabling apoptosis in the presence of Beclin-1did not alter the normal remodeling of the uterus and gland development. Importantly, restoring only Beclin-1-driven autophagy, but not apoptosis, promoted normal uterine remodeling.
“After birth, the uterus goes through a multistep process of development that is required for successful pregnancies in the future. Any deficiencies along this process can have a ripple effect on the ability to conceive and for the pregnancy to succeed,” Kommagani said.
We discovered that one of the steps involves Beclin-1-mediated autophagy, which acts as a molecular switch that governs uterine reprograming by maintaining the endometrial progenitor stem cells that support uterine remodeling.”
The findings have encouraged the researchers to explore developing practical applications both in the field of contraceptives and infertility.
Other contributors to this work include Pooja Popli, Suni Tang, Sangappa B. Chadchan, Chandni Talwar, Edmund B. Rucker III, Xiaoming Guan, Diana Monsivais, John P. Lydon, Christina L. Stallings and Kelle H Moley. The authors are affiliated with Baylor College of Medicine, University of Kentucky or Washington University School of Medicine.
This work was funded, in part, by National Institutes of Health/National Institute of Child Health and Human Development (grants R01HD102680, R01HD104813, 454 R01HD065435, R01HD105800). A Next Gen Pregnancy Award (NGP10125) from the Burroughs Wellcome Fund, NIH Awards NIAID R01 AI132697 and U19 AI142784, the Cytometry and Cell Sorting Core at Baylor College of Medicine with funding from the CPRIT Core Facility Support Award (CPRIT-RP180672) and the NIH (CA125123 and RR024574).
