Naturally occurring antibodies recognize many different noroviruses

Each year in the United States circulating strains of the human norovirus are responsible for approximately 20 million cases of acute gastroenteritis. Hallmark symptoms include severe abdominal cramping, diarrhea and vomiting.

Several vaccine candidates are in clinical trials but it is unclear how effective they will be, given the periodic emergence of novel norovirus variants. Developing broadly effective vaccines will require an understanding of the genetic diversity of the virus and the mechanisms by which the immune system can neutralize it.

Taking a big step forward in that direction, researchers at Baylor College of Medicine and Vanderbilt University Medical Center (VUMC) have isolated a panel of human monoclonal antibodies from subjects with a history of acute gastroenteritis that are cross-reactive and which neutralize a broad range of norovirus variants in laboratory tests.

The study describes a conserved, antigenic site or location on the norovirus that could be used to reformulate vaccine candidates so that they are broadly effective against circulating viral strains. The monoclonal antibodies also could be used to treat or prevent norovirus infection directly or as diagnostic reagents, they added.

Leading the research were the paper’s corresponding authors, Dr. B.V. Venkataram Prasad, the Alvin Romansky Chair in Biochemistry at Baylor and Dr. James Crowe Jr, director of the Vanderbilt Vaccine Center, in collaboration with Dr. Mary Estes, Cullen Chair and professor of molecular virology and microbiology at Baylor.

First authors of the paper were Wilhelm Salmen, a graduate student in the Prasad lab, and Dr. Gabriela Alvarado, formerly of the Crowe lab, now at the National Institute of Allergy and Infectious Diseases.

Unexpected findings

“We were surprised to find naturally occurring antibodies that recognized so many different noroviruses,” said Crowe, the Ann Scott Carell Chair and professor of pediatrics and pathology, microbiology & immunology at VUMC.

“Previously, many experts thought that this would not be possible because of the extreme sequence diversity in the various groups and types of noroviruses in circulation,” he said. “The human immune system continues to surprise us in its capacity to recognize diverse virus variants.”

“One of the fascinating aspects of this study was the unexpected finding of where the human antibody attacks the virus for neutralization,” Prasad said.

“It is exciting to now have human monoclonal antibodies that neutralize many norovirus variants,” added Estes.

Find all the details of this study in the journal Nature Communications.

Also contributing to the work were Khalil Ettayebi and Dr. Liya Hu, assistant professor at Baylor College of Medicine, and Dr. Banumathi Sankaran, from the Lawrence Berkeley National Laboratory in Berkeley, California.

The research was supported in part by the National Institutes of Health and the Welch Foundation of Houston, Texas.

 

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